Investigation of DNA Strand Breaks Induced by <SUP>7</SUP>Li and <SUP>12</SUP>C Ions

SUI Li; ZHAO Kui; NI Mei-Nan; GUO Ji-Yu; LUO Hong-Bing; MEI Jun-Ping; LU Xiu-Qin; ZHOU Ping

Chinese Physics C> 2004, Vol. 28> Issue(10) : 1126-1130

Investigation of DNA Strand Breaks Induced by ⁷Li and ¹²C Ions

China Institute of Atomic Energy,Beijing 102413,China2 The Key Laboratory of Beam Technology and Material Modification of Ministry of Education,Beijing Normal University,Beijing 100875,China3 College of Agronomy,Hunan Agricultural University,Changsha 410128,China4 Hebei University of Technology,Tianjin 300130,China

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Abstract：
Deoxyribonucleic acid (DNA) is an important biomacromolecule. It is a carrier of genetic information and a critical target for radiobiological effects. Numerous lesions have been identified in irradiated DNA. DNA double strand breaks (DSBs) are considered as the most important initial damage of all biological effects induced by ionizing radiation. In this experiment,DNA DSBs induced by heavy ions in the early period were investigated with atomic force microscopy (AFM). Choosing ⁷Li and ¹²C heavy ions with different LET values delivered by HI 13 tandem accelerator respectively,purified plasmid DNA samples in aqueous solution were irradiated at different doses. AFM was used for nanometer level structure analysis of DNA damage induced by these two kinds of heavy ions. Measurement of the DNA fragment lengths was accomplished with the Scion Image analyzed software. Change laws of three forms of DNA,supercoil,open circular and linear form as dose increased were obtained. Distributed function of DNA fragment length was also obtained,and fitted with Tsallis entropy statistical theory.

References

[1]

. XIA Shou-Xuan et al. Radiobiology. Beijing: The Academy of Military Medical Sciences Press, 1998. 65(in Chhinese)(夏寿萱等. 放射生物学. 北京: 军事医学科学院出版社, 1998. 65)2. YANG Chui-Xu, MEI Man-Tong. Space Radiobiology. Guangzhou: Zhongshan University Press, 1995. 23(in Chhinese)(杨垂绪, 梅曼彤. 太空放射生物学. 广州: 中山大学出版社, 1995, 23)3. YUAN Xiong, YE Chang-Qing, ZHOU Ping-Kun. Space Medicine Medical Engineering, 1997, 10(4): 309(in Chhinese)(袁雄, 叶常青, 周平坤. 航天医学与I学工程, 1997, 10(4): 309)4. Hoglund E et al. Int. J. Radiat. Biol., 2000, 76(4): 5395. Newman H C et al. Int. J. Radiat. Biol, 1997, 71(4): 3476. Pang D et al. Scanning Microscopy, 1996, 10(4): 11057. Pang D et al. Radiat. Res., 1998, 150: 6128. Murakami M, Hirokawa H, Hayat a I. J. Biophys. Methods, 2000, 44: 319. Boichot S et al. Radiation Protection Dosimetry, 2002, 99: 14310. Sotolongo-Costa O,Rodriguez A H, Rodgers G J. Entropy, 2000, 2: 172; www.mdpi.org/entropy/11. Harremoes P,Topse F. Entropy, 2001, 3: 191; www.mdpi.org/entropy/

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[11]	LIU Guan-Hua , ZHAN Wen-Long , ZHU Yong-Tai , GUO Zhong-Yan , HU Xiao-Qing , XI Hong-Fei , ZHOU Jian-Qun , YIN Shu-Zhi , FENG En-Pu , ZHAO You-Xiong , WEI Zhi-Yong , FAN En-Jie . Projectile Fragmentation in 46.7MeV/u ¹²C Induced Reactions. Chinese Physics C, 1993, 17(2): 166-172.
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[13]	LIU Guo-Xing , CHEN Ke-Liang , WANG Su-Fang , CAI Wei . ANGULAR DISTRIBUTIONS OF FISSION FRAGMENTS IN INTERACTIONS OF ¹²C IONS WITH VARIOUS TARGETS. Chinese Physics C, 1988, 12(3): 379-385.
[14]	LIU Guo-Xing , CHEN Ke-Liang , MA Hong-Fan , WANG Su-Fang , CAI Wei . FISSION EXCITATION FUNCTION IN INTERATIONS OF ¹²C IONS WITH VARIOUS TARGETS. Chinese Physics C, 1986, 10(5): 597-604.
[15]	XIE Yuan-Xiang , WU Guo-Hua , ZHU Yong-Tai , MIAO Rong-Zhi , FENG En-Pu , YIN Xu , MIAO He-Bin , CAI Jing-Xiang , SHEN Wen-Qing , SUN Shu-MiNG , WEI Jian-Jun , LI Song-Lin . MEASUREMENT AND ANALYSIS OF EMITTED α PARTICLES IN REACTIONS OF ¹²C BOMBARDING ¹²C,²⁷Al AND ^natCa. Chinese Physics C, 1985, 9(1): 71-76.
[16]	WU ZHONG-LI , ZHU YONG-TAI , XIA GUO-ZHONG , LIU BU-SHENG , LI FA-WEI , FAN GUO-YING . QUASI-ELASTIC AND DEEP INELASTIC REACTIONS OF ¹²C ON ²⁷Al. Chinese Physics C, 1981, 5(3): 364-371.
[17]	LI QING-RUN , CHEN SHENG-ZHONG , ZHAO EN-GUANG . ELASTIC π^--¹²C SCATTERING IN THE α-PARTICLE MODEL. Chinese Physics C, 1981, 5(6): 641-647.
[18]	HAN WEN-SHU , LU ZHAO-QI , ZHUANG FEI , ZHENG YU-MING . THE MOMENTUM DISTRIBUTION OF THE NUCLEAR CLUSTER FOR ^6,7Li NUCLEI. Chinese Physics C, 1981, 5(4): 489-492.
[19]	Nuclear Chemistry Group , Nuclear Physics Research Laboratory . RADIOCHEMICAL INVESTIGATION OF MASS DISTRIBUTIONS IN ¹²C-INDUCED FISSION OF ²⁰⁹Bi AND ²³⁸U. Chinese Physics C, 1978, 2(4): 343-349.
[20]	HAN WEN-SHU , CHUANG FI , HE IN . STUDY OF THE MECHANISM OF ¹²C（d,d）¹²C AND ¹²C（d,p）¹³C^* REACTIONS. Chinese Physics C, 1978, 2(3): 249-262.

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SUI Li, ZHAO Kui, NI Mei-Nan, GUO Ji-Yu, LUO Hong-Bing, MEI Jun-Ping, LU Xiu-Qin and ZHOU Ping. Investigation of DNA Strand Breaks Induced by ⁷Li and ¹²C Ions[J]. Chinese Physics C, 2004, 28(10): 1126-1130.

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Received: 2003-12-30
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Investigation of DNA Strand Breaks Induced by ⁷Li and ¹²C Ions

Corresponding author: SUI Li,

China Institute of Atomic Energy,Beijing 102413,China2 The Key Laboratory of Beam Technology and Material Modification of Ministry of Education,Beijing Normal University,Beijing 100875,China3 College of Agronomy,Hunan Agricultural University,Changsha 410128,China4 Hebei University of Technology,Tianjin 300130,China

Received Date: 2003-12-30

Accepted Date: 1900-01-01

Available Online: 2004-10-05

Abstract: Deoxyribonucleic acid (DNA) is an important biomacromolecule. It is a carrier of genetic information and a critical target for radiobiological effects. Numerous lesions have been identified in irradiated DNA. DNA double strand breaks (DSBs) are considered as the most important initial damage of all biological effects induced by ionizing radiation. In this experiment,DNA DSBs induced by heavy ions in the early period were investigated with atomic force microscopy (AFM). Choosing ⁷Li and ¹²C heavy ions with different LET values delivered by HI 13 tandem accelerator respectively,purified plasmid DNA samples in aqueous solution were irradiated at different doses. AFM was used for nanometer level structure analysis of DNA damage induced by these two kinds of heavy ions. Measurement of the DNA fragment lengths was accomplished with the Scion Image analyzed software. Change laws of three forms of DNA,supercoil,open circular and linear form as dose increased were obtained. Distributed function of DNA fragment length was also obtained,and fitted with Tsallis entropy statistical theory.

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Investigation of DNA Strand Breaks Induced by ⁷Li and ¹²C Ions

Investigation of DNA Strand Breaks Induced by ⁷Li and ¹²C Ions