Effect of Alamethicin on the Structure of Lipid Bilayers

LI Da-Peng; HU Shu-Xin; MAI Zhen-Hong; LI Ming

Chinese Physics C> 2005, Vol. 29> Issue(S1) : 1-3

Effect of Alamethicin on the Structure of Lipid Bilayers

Institute of Physics,Chinese Academy of Sciences,Beijing 100080,China

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Abstract：
Alamethicin is a 20-amino acid membrane-active peptide from the fungus Trichoderma viride that spontaneously inserts into lipid membranes and self-associates into transmembrane channels. It has an antimicrobial activity against fungi and Gram-positive bacteria. We have investigated the effect of the peptide on the structure of lipid membranes by X-ray scattering method. The highly aligned stacks of membranes were prepared by evaporation of organic solutions of alamethicin and 1,2-dioleoyl-sn-glycero-3-phosphocholine deposited on clean silicon surfaces and were immersed in excess ultra pure water. The in-situ X-ray measurements show that the membranes with inserted peptides become thinner and softer at high molar peptide/lipid ratio.

References

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LI Da-Peng, HU Shu-Xin, MAI Zhen-Hong and LI Ming. Effect of Alamethicin on the Structure of Lipid Bilayers[J]. Chinese Physics C, 2005, 29(S1): 1-3.

LI Da-Peng, HU Shu-Xin, MAI Zhen-Hong and LI Ming. Effect of Alamethicin on the Structure of Lipid Bilayers[J]. Chinese Physics C, 2005, 29(S1): 1-3. shu

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Received: 2005-10-13
Revised: 1900-01-01

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Effect of Alamethicin on the Structure of Lipid Bilayers

Corresponding author: LI Ming,

Institute of Physics,Chinese Academy of Sciences,Beijing 100080,China

Received Date: 2005-10-13

Accepted Date: 1900-01-01

Available Online: 2005-01-02

Abstract: Alamethicin is a 20-amino acid membrane-active peptide from the fungus Trichoderma viride that spontaneously inserts into lipid membranes and self-associates into transmembrane channels. It has an antimicrobial activity against fungi and Gram-positive bacteria. We have investigated the effect of the peptide on the structure of lipid membranes by X-ray scattering method. The highly aligned stacks of membranes were prepared by evaporation of organic solutions of alamethicin and 1,2-dioleoyl-sn-glycero-3-phosphocholine deposited on clean silicon surfaces and were immersed in excess ultra pure water. The in-situ X-ray measurements show that the membranes with inserted peptides become thinner and softer at high molar peptide/lipid ratio.

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Effect of Alamethicin on the Structure of Lipid Bilayers

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