Quantitative Analysis of Iron in Protein Bands Existing in Polyacrylamide Gel Matrix with Synchrotron Radiation

LIU Ying-Bin; GAO Yu-Xi; CHEN Chun-Ying; LI Bai; YU Hong-Wei; WANG Jiang-Xue; HE Wei; HUANG Yu-Ying; CHAI Zhi-Fang

Chinese Physics C> 2005, Vol. 29> Issue(S1) : 61-64

Quantitative Analysis of Iron in Protein Bands Existing in Polyacrylamide Gel Matrix with Synchrotron Radiation

Institute of High Energy Physics,CAS,Beijing 100049,China2 Second Affiliated Hospital of Zhejiang University,Hangzhou 310009,China

Abstract
HTML
Reference
Related

PDF

Abstract：
A procedure has been proposed for quantification of Fe in protein bands in polyacrylamide gel matrix with synchrotron radiation X-ray fluorescence(SRXRF)analysis. Human hemoglobin was separated with isoelectric focusing (IEF), and Fe amounts in protein bands were measured with SRXRF. Calibration standards were prepared by electrophoresis of bovine serum albumin containing known amounts of Fe with stacking gel. The calibration curves can be obtained in a range of 0—5.1ng Fe and linear relationship between dosage of metals and fluorescent intensity can be observed (R²=0.9897). The method provides detection limit of 0.63ng Fe,and the recovery of Fe determination is about 106%. The hyphenated technique of SRXRF and IEF has advantages of quantifying trace elements in proteins and identifying the proteins simultaneously. It can be employed for speciation analysis of trace elements in biological samples.

References

[1]

. Hiroki H J. Anal. At. Spectrom., 2004, 19: 5—142. Langevelde F, Vis R D. Anal. Chem., 1991, 63: 2253—22593. Geraki K, Farquharson M J, Bradley D A. Phys. Med. Biol.,2002, 47: 2327—23394. Ide-Ektessabi A, Fujisawa S, Sugimura K et al. X-Ray Spec-trom., 2002, 31: 7—115. Twining B S, Baines S B, Fisher N S et al. Anal. Chem.,2003, 75: 3806—38166. Ortega R, Bohic S, Tucoulou R et al. Anal. Chem., 2004,76: 309—3147. CHEN C Y, ZHANG P Q, CHAI Z F et al. Science in China(English version). 2000, 43: 88—928. GAO Y X, CHEN C Y, CHAI Z F et al. Analyst, 2002,127: 1400—14049. GAO Y X, CHEN C Y, ZHANG P Q et al. Anal. Chem.Acta, 2003, 485: 131—13710. GAO Yu-Xi, CHEN Chun-Ying, ZHAO Jiu-Jiang et al.Chinese J. Anal. Chem., 2003, 31: 395—398(in Chinese)(高愈希，陈春英，赵九江等.分析化学,2003, 31: 395-398)11. GAO Y X, LIU Y B, CHEN C Y et al. J. Anal. At. Spec-trom., 2005, 20: 473—47512. GAO Yu-Xi, FENG Wei-Yue, LI Bai et al. Nuclear Tech-niques, 2004, 27:165—168(in Chinese)(高愈希，丰伟悦，李柏等.核技术，2004, 27:165-168)13. DONG Yuan-Xing, GAO Yu-Xi, CHEN Chun-Ying et al.Chinese J. Anal. Chem.,(in Chinese,Accepted)(董元兴，高愈希，陈春英等.分析化学，已接受)14. Davis B J. Ann. N. Y. Acad. Sci., 1964, 121 404—427

[1]	HUA Wei , LI Zhi-Hong , WU Zi-Yu , WU Zong-Hua , HUANG Yu-Ying . The conformational changes of encapsulated cytochrome c in SiO2 sol-gel materials. Chinese Physics C, 2005, 29(S1): 12-15.
[2]	LIU Lei , CHU Wang-Sheng , ZHONG Jun , MA Si-Xuan , GAO Bin , WU Zi-Yu , GAN Li-Hua , SHU Chun-Ying , WANG Chun-Ru . Preparation and EXAFS Study of Endohedral Metallofulleren Y@C₈₂. Chinese Physics C, 2005, 29(S1): 102-105.
[3]	FENG Liu-Xing , XIAO Hai-Qing , HE Xiao , ZHANG Zhi-Yong , LIU Nian-Qing , ZHAO Yu-Liang , HUANG Yu-Ying . Spatial Distribution of Elements in the Brain of Rats Measured by Synchrotron Radiation X-ray Fluoresence. Chinese Physics C, 2005, 29(10): 1012-1016.
[4]	HAO Hu-Lin , FENG Ying , HUANG Yu-Ying , TIAN Sheng-Ke , LU Ling-Li , YANG Xiao-E , WEI You-Zhang . In Situ Analysis of Cellular Distribution of Iron and Zinc in Rice Grain Using SRXRF Method. Chinese Physics C, 2005, 29(S1): 56-60.
[5]	GONG Yan , CHEN Bo , NI Qi-Liang , CUI Ming-Qi , ZHAO Yi-Dong , WU Zhong-Hua . Thickness Analysis for Thin-Film by Glazing Exit X-ray Fluorescence With Synchrotron Radiation Source. Chinese Physics C, 2005, 29(11): 1104-1106.
[6]	XIANG Dao , HUANG Wen-Hui , HE Xiao-Zhong . Impact of Coherent Synchrotron Radiation on Beam Qualities in a Chicane. Chinese Physics C, 2004, 28(5): 555-559.
[7]	YU Fu-Sheng , YUAN Wan-Ming , HAN Song , JIN Ke , HUANG Yu-Ying , HE Wei , DONG Jin-Quan , JIA Xiu-Qin , CAO Jie , WANG Hong-Yue . Measurements of Trace Elements in Melt Inclusions by Synchrotron Radiation X-Ray Fluorescence Microprobe Techniques. Chinese Physics C, 2004, 28(6): 675-678.
[8]	LI Shu-Jun , ZHAO Wei , CHU Wang-Sheng , NIU Li-Wen , TENG Mai-Kun , HU Tian-Dou , XIE Ya-Ning , WU Zi-Yu . XAS Study of a Venom Metalloprotein. Chinese Physics C, 2003, 27(S1): 19-22.
[9]	ZHANG Zhi-Yong , LIU Nian-Qing , LI Fu-Liang , HUANG Yu-Ying , HE Wei , Lei Hao , ZHOU Zhen-You . The Manganese Distribution in Olfactory Bulbs of Rat Measured by Synchrotron Radiation X-ray Fluorescence. Chinese Physics C, 2003, 27(S1): 114-117.
[10]	TANG Yun-Hui , YUAN Wan-Ming , HAN Chun-Ming , HUANG Yu-Ying , HE Wei . SRXRF analysis on muscovite in Yuerya gold deposit, Eastern Hebei, China. Chinese Physics C, 2003, 27(S1): 109-113.
[11]	SUN Ke-Xu , YI Rong-Qing , HUANG Tian-Xuan , CUI Yan-Li , YANG Jia-Min , JIANG Shao-En , DING Yong-Kun , ZHANG Bao-Han , WANG Hong-Bin , WEN Shu-Huai , ZHENG Zhi-Jian , CUI Ming-Qi , ZHU Pei-Ping , ZHAO Yi-Dong , LI Gang , TANG E-Sheng . Applications for Synchrotron Radiation in Inertial Confinement Fusion Research. Chinese Physics C, 2001, 25(S1): 121-127.
[12]	JU Xin , SHEN Xian-Sheng , KANG Shi-Xiu , WU Zi-Qin , SUN Li-Guang , ZHANG Li , YIN Xue-Bin , HUANG Yu-Ying . X-Ray Fluorescence Analysis of Some Plants in the King George Island,Antarctica. Chinese Physics C, 2001, 25(S1): 44-48.
[13]	KANG Shi-Xiu , SUN Xia , YAO Kun , WU Zi-Qin , HUANG Yu-Ying , JU Xin , XIAN Ding-Chang . Measurement and Calculation of Escape Peak in SR-XRF Analysis. Chinese Physics C, 2001, 25(S1): 39-43.
[14]	ZHENG Shu-Xin , CUI Yu-Peng , CHEN Huai-Bi , XIAO Li-Ling . Quantitative Analysis of Coupler Tuning. Chinese Physics C, 2001, 25(1): 61-68.
[15]	ZHAO YiDong , CUI MingQi , ZHU Peiping , CUI CongWu . Application of Transmission Grating in Diagnoses of Synchrotron Radiation Beamline Output. Chinese Physics C, 2000, 24(11): 1043-1049.
[16]	Zhao Limin , Wu Yingrong , Huang Yuying , Li Guangcheng , Jia Quanjie , Yuan Lizhen , Chen Jiapen . Application of Total Reflection X-ray Fluorescence Analysis in Bionmedical Field. Chinese Physics C, 1998, 22(2): 186-191.
[17]	Wu Yingrong , Chao Zhiyu , Pan Juxiang , Hong Rong , Xiao Yanan , Li Guangcheng , Huang Yuying , Zhao Limin . Microprobe X-Ray Fluoresceoce Experintental Station of Beijing Syochrotron Radiation Facility. Chinese Physics C, 1997, 21(5): 475-480.
[18]	Jiang Haiyin , Wang Dewu , Liu Jianfei , Wu Zhonghua , Xie Yahing , Gu Mu , Chen Lingyan . Study of Fluorescent Decay Properties of Fluorides With Beijing Synchrotron Radiation Source. Chinese Physics C, 1996, 20(12): 1103-1109.
[19]	Yan Xiunling , Jiang Zhijin , Li Zuohong . Quantitative Analysis for U(1) Gauge Theory. Chinese Physics C, 1995, 19(6): 527-533.
[20]	ZHANG Ge , ZHU Shan-Gen , ZHOU Yong-Shen , ZHAO Meng , ZHAO Di-Xin , LI Jia , HAN Shi-Wen , FU Zheng-Shan . Magnetic Axis Measurement in the Solenoid of Beijing Spectrometer. Chinese Physics C, 1991, 15(3): 200-206.

Access

Get Citation

LIU Ying-Bin, GAO Yu-Xi, CHEN Chun-Ying, LI Bai, YU Hong-Wei, WANG Jiang-Xue, HE Wei, HUANG Yu-Ying and CHAI Zhi-Fang. Quantitative Analysis of Iron in Protein Bands Existing in Polyacrylamide Gel Matrix with Synchrotron Radiation[J]. Chinese Physics C, 2005, 29(S1): 61-64.

RIS(for EndNote,Reference Manager,ProCite)

BibTex

Txt

Milestone

Received: 2005-10-28
Revised: 1900-01-01

Article Metric

Article Views(3079)
PDF Downloads(688)
Cited by(0)

Policy on re-use

To reuse of subscription content published by CPC, the users need to request permission from CPC, unless the content was published under an Open Access license which automatically permits that type of reuse.

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Quantitative Analysis of Iron in Protein Bands Existing in Polyacrylamide Gel Matrix with Synchrotron Radiation

Corresponding author: GAO Yu-Xi,

Institute of High Energy Physics,CAS,Beijing 100049,China2 Second Affiliated Hospital of Zhejiang University,Hangzhou 310009,China

Received Date: 2005-10-28

Accepted Date: 1900-01-01

Available Online: 2005-01-02

Abstract: A procedure has been proposed for quantification of Fe in protein bands in polyacrylamide gel matrix with synchrotron radiation X-ray fluorescence(SRXRF)analysis. Human hemoglobin was separated with isoelectric focusing (IEF), and Fe amounts in protein bands were measured with SRXRF. Calibration standards were prepared by electrophoresis of bovine serum albumin containing known amounts of Fe with stacking gel. The calibration curves can be obtained in a range of 0—5.1ng Fe and linear relationship between dosage of metals and fluorescent intensity can be observed (R²=0.9897). The method provides detection limit of 0.63ng Fe,and the recovery of Fe determination is about 106%. The hyphenated technique of SRXRF and IEF has advantages of quantifying trace elements in proteins and identifying the proteins simultaneously. It can be employed for speciation analysis of trace elements in biological samples.

HTML

Reference (1)

Quantitative Analysis of Iron in Protein Bands Existing in Polyacrylamide Gel Matrix with Synchrotron Radiation

Abstract：

References

Access

Article Metrics

Metrics

通讯作者: 陈斌, bchen63@163.com

Email This Article