Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering

HONG Xin-Guo; WENG Yu-Xiang; LI Ming

Chinese Physics C> 2003, Vol. 27> Issue(S1) : 57-62

Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering

Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100039,China2 Laboratory of Soft Matter Physics,Institute of Physics,Chinese Academy of Sciences，Beijing l00080,China

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Abstract：
The low-resolution shape of the integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria Rhodobacter sphaeroides 2.4. 1 in detergent solution has been determined from synchrotron small-angle X-ray scattering (SAXS) data using directly curve fitting by an ellipsoid and ab initio shape determination method or multipole expansion respectivelv. The results indicate that the LH2 proteins in aoueous solution has a shape of oblate plate. In contrast to the perfect symmetrical ring-like structure of LH2 in crystalline, the molecular shape of the LH2 complex in detergent solution clearly deviates from the ring-like crystal structure, which is consistent with the result of single molecular spectroscopy study of the isolated single LH2 molecules.

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HONG Xin-Guo, WENG Yu-Xiang and LI Ming. Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering[J]. Chinese Physics C, 2003, 27(S1): 57-62.

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Received: 2003-11-21
Revised: 1900-01-01

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沈阳化工大学材料科学与工程学院沈阳 110142

Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering

Corresponding author: HONG Xin-Guo,

Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100039,China2 Laboratory of Soft Matter Physics,Institute of Physics,Chinese Academy of Sciences，Beijing l00080,China

Received Date: 2003-11-21

Accepted Date: 1900-01-01

Available Online: 2003-01-02

Abstract: The low-resolution shape of the integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria Rhodobacter sphaeroides 2.4. 1 in detergent solution has been determined from synchrotron small-angle X-ray scattering (SAXS) data using directly curve fitting by an ellipsoid and ab initio shape determination method or multipole expansion respectivelv. The results indicate that the LH2 proteins in aoueous solution has a shape of oblate plate. In contrast to the perfect symmetrical ring-like structure of LH2 in crystalline, the molecular shape of the LH2 complex in detergent solution clearly deviates from the ring-like crystal structure, which is consistent with the result of single molecular spectroscopy study of the isolated single LH2 molecules.

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Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering

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