Solution conformation of the response regulator proteins from Deinococcus radiodurans studied by SAXS

LI Li-Qin; LIU Ying; LIU Peng; DONG Yu-Hui

doi:10.1088/1674-1137/35/10/017

Chinese Physics C> 2011, Vol. 35> Issue(10) : 974-977 DOI: 10.1088/1674-1137/35/10/017

Solution conformation of the response regulator proteins from Deinococcus radiodurans studied by SAXS

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In this paper the solution conformation of the response regulator proteins from Deinococcus radiodurans was studied by small-angle X-ray scattering (SAXS). The SAXS curves of Dr-rrA in solutions were obtained at Beamline 1W2A of Beijing Synchrotron Radiation Facility (BSRF). Two possible conformations of the response regulator proteins, compact and incompact conformations, have been represented by the known crystallographic structures. And theoretical solution scattering curves of the two possible conformations were calculated and fitted to the experimental scattering curve of Dr-rrA, respectively. The result indicates that the solution conformation of the response regulator proteins is inclined to the compact one, which is in agreement with the result of biochemical experiments.

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LI Li-Qin, LIU Ying, LIU Peng and DONG Yu-Hui. Solution conformation of the response regulator proteins from Deinococcus radiodurans studied by SAXS[J]. Chinese Physics C, 2011, 35(10): 974-977. doi: 10.1088/1674-1137/35/10/017

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Received: 2010-12-24
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Solution conformation of the response regulator proteins from Deinococcus radiodurans studied by SAXS

Received Date: 2010-12-24

Accepted Date: 2011-02-11

Available Online: 2011-10-05

Abstract: In this paper the solution conformation of the response regulator proteins from Deinococcus radiodurans was studied by small-angle X-ray scattering (SAXS). The SAXS curves of Dr-rrA in solutions were obtained at Beamline 1W2A of Beijing Synchrotron Radiation Facility (BSRF). Two possible conformations of the response regulator proteins, compact and incompact conformations, have been represented by the known crystallographic structures. And theoretical solution scattering curves of the two possible conformations were calculated and fitted to the experimental scattering curve of Dr-rrA, respectively. The result indicates that the solution conformation of the response regulator proteins is inclined to the compact one, which is in agreement with the result of biochemical experiments.

Solution conformation of the response regulator proteins from Deinococcus radiodurans studied by SAXS

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Solution conformation of the response regulator proteins from Deinococcus radiodurans studied by SAXS

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